High-performance liquid chromatography on silica modified with temperature-responsive polymers

The separation selectivity of temperature-responsive poly (N-isopropylacrylamide)-modified silica as a packing material for high performance liquid chromatography was investigated with steroids, alkaloids, and substituted anilines as solutes. The elution profiles of the solutes depended on the temperature of the column and the methanol content of the mobile phase, indicating that the separation selectivity could be controlled by the column temperature or the mobile phase composition.