Journal
INTENSIVE CARE MEDICINE
Volume 27, Issue 8, Pages 1269-1273Publisher
SPRINGER-VERLAG
DOI: 10.1007/s001340100981
Keywords
polymerase chain reaction; sepsis; intensive care
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Objective: To confirm the sensitivity of the polymerase chain reaction PCR) technique (versus blood cultures) and to gain a better understanding of the incidence of true- and false-positive results when using this technique. Design: Observational study. Setting: Fourteen-bed, level 3 intensive care unit. Patients: Hundred twenty-six critically ill adult patients. Hundred ninety-seven blood culture and PCR samples taken as clinically indicated for suspected sepsis, according to routine ICU protocol. Measurements and results: The PCR product (16SrDNA: 341F-1195R) was sequenced and compared with a database of known species (Gene-bank) to identify the bacterial nucleic acid. The PCR or blood culture result was classified as a true-positive if there was other microbiological or clinical supporting evidence.
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