Journal
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
Volume 8, Issue -, Pages 186-191Publisher
BEILSTEIN-INSTITUT
DOI: 10.3762/bjoc.8.20
Keywords
biotransformations; cofactor regeneration; green chemistry; hydroxylation; P450-monooxygenase
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Funding
- German Federal Environmental Foundation (Deutsche Bundesstiftung Umwelt, DBU) within the DBU-network ChemBioTec [AZ 13234-32]
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The hydroxylation of n-alkanes, which proceeds in the presence of a P450-monooxygenase advantageously at temperatures significantly below room temperature, is described. In addition, an enzymatic hydroxylation of the liquid gas n-butane with in situ cofactor regeneration, which does not require high-pressure conditions, was developed. The resulting 2-butanol was obtained as the only regioisomer, at a product concentration of 0.16 g/L.
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