Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 925, Issue 1-2, Pages 49-67Publisher
ELSEVIER
DOI: 10.1016/S0021-9673(01)01009-3
Keywords
stationary phases, LC; retention mechanism; pH effects; silanol activity; ion exclusion; hybrid packings
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In reversed-phase chromatography, the retention of ionizable analytes is influenced by the ionic properties of the packing caused by surface silanol groups. We have measured the ion-exchange properties of both reversed-phase bonded phases and their underlying base materials. The probe used in this part of the study was bretylium tosylate. The acquired knowledge is then used for a complete and quantitative understanding of the retention behavior of ionizable compounds as a function of the pH of the mobile phase and the solvent composition. We have studied the retention pattern of a broad range of acids, bases, and polyfunctional analytes over the pH range from 2 to 11 and from water to 80% acetonitrile. A few application examples demonstrate the relevant findings. (C) 2001 Elsevier Science B.V. All rights reserved.
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