Journal
FEBS LETTERS
Volume 503, Issue 1, Pages 7-12Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(01)02625-4
Keywords
cell deaths; apoptosis; ceramide; sphingomyelinase; ceramide synthase
Funding
- NIGMS NIH HHS [GM 43825] Funding Source: Medline
Ask authors/readers for more resources
Ceramide accumulation in the cell can occur from either hydrolysis of sphingomyelin or by de novo synthesis. In this study, we found that blocking de novo ceramide synthesis significantly inhibits ceramide accumulation and subsequent cell death in response to tumor necrosis factor alpha. When cells were pre-treated with glutathione, a proposed cellular regulator of neutral sphingomyelinase, inhibition of ceramide accumulation at early time points was achieved with attenuation of cell death. Inhibition of both pathways achieved near-complete inhibition of ceramide accumulation and cell death indicating that both pathways of ceramide generation are stimulated. This illustrates the complexity of ceramide generation in cytokine action. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available