Journal
DEVELOPMENTAL BIOLOGY
Volume 236, Issue 2, Pages 411-420Publisher
ACADEMIC PRESS INC
DOI: 10.1006/dbio.2001.0345
Keywords
hedgehog; Ci; Fu; Su(fu); signal transduction; nuclear import; pattern formation
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Funding
- NIEHS NIH HHS [ES 10549] Funding Source: Medline
- NIGMS NIH HHS [GM 057450] Funding Source: Medline
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Much of our understanding of the Hedgehog (Hh) signaling pathway comes from Drosophila, where a gradient of Hh signaling regulates the function of the transcription factor Cubitus interruptus (Ci) at three levels: protein stabilization, nuclear import, and activation. Regulation of Ci occurs in a cytoplasmic complex containing Ci, the kinesin-like protein Costal-2 (Cos2), the serine-threonine kinase Fused (Fu), and the Suppressor of Fused [Su(fu)] protein. The mechanisms by which this complex responds to different levels of Hh signaling and establishes distinct domains of gene expression are not fully understood. By sequentially mutating components from the Ci signaling complex, their roles in each aspect of Ci regulation can be analyzed. The Cos2-Ci core complex is able to mediate Hh-regulated activation of Ci but is insufficient to regulate nuclear import and cleavage. Addition of Su(fu) to the core complex blocks nuclear import while the addition of Fu restores Hh regulation of Ci nuclear import and proteolytic cleavage. Fu participates in two partially redundant pathways to regulate Ci nuclear import; the kinase function plays a positive role by inhibiting Su(fu), and the regulatory domain plays a negative role in conjunction with Cos2. (C) 2001 Academic Press.
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