Journal
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
Volume 1527, Issue 3, Pages 156-160Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0304-4165(01)00161-1
Keywords
X-ray absorption spectroscopy; isothiocyanate; sinigrin; myrosinase
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Funding
- NIGMS NIH HHS [GM57375] Funding Source: Medline
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Sulfur K-edge X-ray absorption spectroscopy has been used to determine the chemical identity of the sulfur-containing species in horseradish (Armoracia lapthifolia) and wasabi (Wasabia japonica) in situ, before and after cell disruption. The major sulfur-containing species in the intact root is sinigrin (1-thio-beta -D-glucopyranose 1-N-(sulfoxy)-3-buteneimidate) and related congeners. Disrupting the cells by applying local pressure allowed the conversion of the sulfur moieties in sinigrin to isothiocyanates and sulfate in approximately equimolar amounts. In contrast to previous suggestions, no detectable thiocyanates were formed, but an unusual thio intermediate may have been identified for the first time. (C) 2001 Elsevier Science B.V. All rights reserved.
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