Journal
EMBO JOURNAL
Volume 20, Issue 16, Pages 4341-4348Publisher
WILEY
DOI: 10.1093/emboj/20.16.4341
Keywords
cGMP; chemotaxis; Dictyostelium; evolution; guanylyl cyclase
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A new Dictyostelium discoideum cyclase gene was identified that encodes a protein (sGC) with 35% similarity to mammalian soluble adenylyl cyclase (sAC). Gene disruption of sGC has no effect on adenylyl cyclase activity and results in a > 10-fold reduction in guanylyl cyclase activity. The scg- null mutants show reduced chemotactic sensitivity and aggregate poorly under stringent conditions. With Mn2+/GTP as substrate, most of the sGC activity is soluble, but with the more physiological Mg2+/GTP the activity is detected in membranes and stimulated by GTP gammaS. Unexpectedly, orthologues of sGC and sAC are present in bacteria and vertebrates, but absent from Drosophila melanogaster, Caenorhabditis elegans, Arabidopsis thaliana and Saccharomyces cerevisiae.
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