Journal
APPLIED PHYSICS LETTERS
Volume 79, Issue 8, Pages 1208-1210Publisher
AMER INST PHYSICS
DOI: 10.1063/1.1397255
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Here, we present an optical technique that can induce waves of calcium ion concentration in live biological cells. Ca2+ waves were induced by femtosecond pulsed-laser illumination. Living HeLa cells were exposed to focused 140 fs pulses of 780 nm wavelength at 30 mW average power. Ca2+ waves were imaged by fluorescence and were observed to propagate from the laser focal point inside the cell. Photoinduced generation of Ca2+ waves can be performed at any point inside the cell, an improvement over previous mechanical or biochemical stimulation techniques. (C) 2001 American Institute of Physics.
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