Journal
JOURNAL OF BIOTECHNOLOGY
Volume 89, Issue 2-3, Pages 99-106Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0168-1656(01)00319-4
Keywords
decolourisation; azo dye; packed bed bioreactor; immobilised; Phanerochaete chrysosporium
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The degradation of an azo dye, Orange II, by immobilised Phanerochaete chrysosporium in a continuous packed bed bioreactor for periods longer than 30 days has been carried out. Nearly complete decolourisation ( > 95%) was achieved when working at a high dye load rate of 0.2 g l(-1) d(-1), a temperature of 37 degreesC, a hydraulic retention time (HRT) of 24 h and applying oxygen gas in a pulsed flow. These conditions allowed Manganese peroxidase (MnP) production and the subsequently Orange II decolourisation. A correlation between residual MnP activity in the effluent and decolourisation was established. Apparently, for decolourisation to be effective, a minimum MnP activity was required, no substantial increase in efficiency at MnP activities higher than 10 U l(-1) was observed. The treatment caused, the breakdown of the chromophoric group as well as the cleavage of the aromatic ring. (C) 2001 Elsevier Science BY. All rights reserved.
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