Journal
ANALYTICA CHIMICA ACTA
Volume 441, Issue 2, Pages 303-308Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0003-2670(01)01112-6
Keywords
cetyltrimethylammonium bromide; nucleic acids; DNA
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After the addition of cetyltrimethylammonium bromide to DNA, the of DNA increased and changed from -70.2 to +21.6 mV, and the absorbance at 260.0 nm increased. This indicates that DNA is partly denatured and a large new ion association complex formed. The formation of the new associate can result in very strong light scattering at 414.0 ran, which is in proportion to the concentration of nucleic acids in the range 2.5 x 10(-8) to 2.0 x 10(-5) g ml(-1) for calf thymus (ct)DNA, 2.5 x 10(-8) to 2.0 x 10(-5) g ml(-1) for fish sperm (fs)DNA, and 5.0 x 10(-8) to 5.0 x 10(-5) g ml(-1) for yeast (y)RNA. The detection limits (3 sigma) are 8.3, 2.4, and 23.4 ng ml(-1), respectively. Synthetic samples were determined satisfactorily. (C) 2001 Elsevier Science B.V. All rights reserved.
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