Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 276, Issue 35, Pages 32437-32445Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M104000200
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- NIAID NIH HHS [AI23259] Funding Source: Medline
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Intracellular Ca2+ in Trypanosoma cruzi is mainly located in an acidic compartment named the acidocalcisome, which among other pumps and exchangers possesses a plasma membrane-type Ca2+-ATPase. Evidence for an endoplasmic reticulum-located Ca2+ uptake has been more elusive and based on indirect results. Here we report the cloning and sequencing of a gene encoding a sarcoplasmic-endoplasmic reticulum-type Ca2+-ATPase from T. cruzi. The protein (TeSCA) predicted from the nucleotide sequence of the gene has 1006 amino acids and a molecular mass of 109.7 kDa. Several sequence motifs found in sarcoplasmic-endoplasmic reticulum-type Ca2+-ATPases were present in TeSCA. Expression of TcSCA in yeast mutants deficient in the Golgi and vacuolar Ca2+ pumps (pmr1 pmc1 cnb 1) restored growth on EGTA. Membranes were isolated from the pmr1 pmc1 cnb1 mutant transformed with TcSCA, and it was found that the TcSCA polypeptide formed a Ca2+-dependent and hydroxylamine-sensitive P-32-labeled. phosphoprotein of 110 kDa in the presence of [gamma-P-32]ATP. Cyclopiazonic acid, but not thapsigargin, blocked this phosphoprotein formation. Transgenic parasites expressing constructs of TcSCA with green fluorescent protein exhibited co-localization of TcSCA with the endoplasmic reticulum proteins BiP and calreticulin. An endoplasmic reticulum location was also found in amastigotes and trypomastigotes using a polyclonal antibody against a COOH-terminal region of the protein. The ability of TcSCA to restore growth of mutant pmr1 pmc1 cnb 1 on medium containing Mn2+ suggests that TcSCA may also regulate Mn2+ homeostasis by pumping Mn2+ into the endoplasmic reticulum of T. cruzi.
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