4.7 Article

A novel method of gene transcript profiling in airway biopsy homogenates reveals increased expression of a Na+-K+-Cl- cotransporter (NKCCl) in asthmatic subjects

Journal

GENOME RESEARCH
Volume 11, Issue 9, Pages 1473-1483

Publisher

COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gr.191301

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Funding

  1. NHLBI NIH HHS [R01 HL-61662] Funding Source: Medline
  2. NIDCR NIH HHS [P01 DE7946] Funding Source: Medline

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Comprehensive and systematic analysis of airway gene expression represents a strategy for addressing the Multiple, complex, and largely untested hypotheses that exist for disease mechanisms, including asthma. Here, we report a novel real-time PCR-based method specifically designed for quantification of multiple low-abundance transcripts using as little as 2.5 fg, of total RNA per gene. This method of gene expression profiling has the same specificity and sensitivity as RT-PCR and a throughput level comparable to low-density DNA microarray hybridization. In this two-step method, multiplex RT-PCR is successfully combined with individual gene quantification via real-time PCR on generated cDNA product. Using this method, we measured the expression of 75 genes in bronchial biopsies from asthmatic versus healthy subjects and found expected increases in expression levels of Th2 cytokines and their receptors in asthma. Surprisingly, we also found increased gene expression of NKCCl-a Na+-K+-Cl- cotransporter. Using immunohistochemical method, we confirmed increased protein expression for NKCCl in the asthmatic subject with restricted localization to goblet cells. These data validate the new transcriptional profiling method and implicate NKCCl in the pathophysiology of mucus hypersecretion in asthma. Potential applications for this method include transcriptional profiling in limited numbers of laser captured cells and validation of DNA microarray data in clinical specimens.

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