Journal
JOURNAL OF BASIC MICROBIOLOGY
Volume 55, Issue 9, Pages 1118-1124Publisher
WILEY-BLACKWELL
DOI: 10.1002/jobm.201400901
Keywords
Adherence; FaeG subunit; F4(+) Enterotoxigenic Escherichia coli
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Funding
- Chinese National Science Foundation [30571374, 30771603, 31072136, 31270171]
- Genetically Modified Organisms Technology Major Project of China [2014ZX08006-001B]
- 948 programme from Ministry of Agriculture of the People's Republic of China [2011-G24]
- Program for ChangJiang Scholars and Innovative Research Team In University PCSIRT [IRT0978]
- Program granted for Scientific Innovation Research of College Graduate in Jiangsu province [KYLX_1359]
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The FaeG subunit is the major constituent of F4(+) fimbriae, associated with glycoprotein and/or glycolipid receptor recognition and majorly contributes to the pathogen attachment to the host cells. To investigate the key factor involved in the fimbrial binding of F4(+)Escherichia coli, both the recombinant E. coli SE5000 strains carrying the fae operon gene clusters that express the different types of fimbriae in vitro, named as rF4ab, rF4ac, and rF4ad, respectively, corresponding to the fimbrial types F4ab, F4ac, and F4ad, and the three isogenic in-frame faeG gene deletion mutants were constructed. The adhesion assays and adhesion inhibition assays showed that faeG mutants had a significant reduction in the binding to porcine brush border as well as the intestinal epithelial cell lines, while the complemented strain faeG/pfaeG restored the adhesion function. The recombinant bacterial strains rF4ab, rF4ac, and rF4ad have the same binding property as wild-type F4(+)E. coli strains do and improvement in terms of binding to porcine brush border and the intestinal epithelial cells, and the adherence was blocked by the monoclonal antibody anti-F4 fimbriae. These data demonstrate that the fimbrial binding of F4(+)E. coli is directly mediated by the major FaeG subunit.
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