Journal
JOURNAL OF FLUORESCENCE
Volume 11, Issue 3, Pages 187-195Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1023/A:1012297000742
Keywords
single-fluorophore imaging; green fluorescent protein; cell membrane; membrane skeleton; oligomer; translational diffusion
Ask authors/readers for more resources
Recently, observation and tracking of single fluorophores, which we term single fluorophore dynamic imaging (SFDI) in this review, in living cells have been achieved. In particular, the recent success of SFDI of individual proteins tagged with green fluorescent protein (GFP) in live cells has opened new important possibilities for studying events occurring in living cells at the level of single molecules. Specifically, SFDI of GFP allows the tracking of movement and oligomerization levels of individual oligomers (monomers) in living cells and, thus, provides powerful means to investigate the movement, assembly, localization, and activation that signaling molecules undergo following an external stimulus. In this short account, we first review technologically important points for SFDI of GFP molecules in living cells, then give examples of its application, and, finally, propose a synergistic use of SFDI and single-particle tracking, a technique used for investigating single or small groups of molecules in live cells over the past 15 years.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available