Journal
INFECTION AND IMMUNITY
Volume 69, Issue 9, Pages 5953-5957Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.69.9.5953-5957.2001
Keywords
-
Categories
Funding
- NIAID NIH HHS [F32 AI010481, AI29619, AI10481, R37 AI029619, R01 AI027655] Funding Source: Medline
Ask authors/readers for more resources
We developed a competitive index assay for murine listeriosis that tests the virulence of Listeria monocytogenes strains in different organs and at various times postinoculation. Studies presented here demonstrate the reproducibility of this assay during primary and secondary infection of inbred and outbred mice. We verified the validity of this assay by performing competitive index analysis of a well-characterized strain of L. monocytogenes lacking the actA gene. In addition, we found that while L. monocytogenes strains unable to recruit vasodilator-stimulated phosphoprotein (VASP) to their surface exhibit a 10-fold virulence attenuation in the livers of naive animals, they display a 50-fold survival defect in the liver during secondary listeriosis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available