Journal
DEVELOPMENTAL DYNAMICS
Volume 222, Issue 1, Pages 120-126Publisher
WILEY-BLACKWELL
DOI: 10.1002/dvdy.1170
Keywords
angiopoietin; capillary; glomerulus; metanephros; PECAM; Tie-2
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Angiopoietins (Ang) are secreted factors which bind the Tie-2 receptor and modulate endothelial growth. This signalling system is known to be expressed in later stages of maturation of the mouse metanephros, the adult kidney precursor. In this study, by using reverse transcription polymerase chain reaction and Northern and Western blotting, we demonstrated that Ang-1, Ang-2, and Tie-2 were expressed during early metanephrogenesis when interstitial and glomerular capillaries begin to form. By using immunohistochemistry, embryonic kidney capillaries in the interstitium and glomeruli expressed Tie-2 at a later stage of differentiation compared with vascular endothelial growth factor receptor-2 and platelet-endothelial cell adhesion molecule. Addition of 200 ng/ml Ang-1 to explanted embryonic day (E) 12.5 metanephroi increased the proportion of vascular glomeruli that formed during 1 week in culture. These results are consistent with the hypotheses that Tie-2 has a role in vascular growth in the early stages of mammalian nephrogenesis and that Tie-2 activation may maintain the integrity of recently formed interstitial and glomerular vessels. (C) 2001 Wiley-Liss, Inc.
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