Journal
ANALYTICAL CHEMISTRY
Volume 73, Issue 17, Pages 4277-4285Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac0101050
Keywords
-
Categories
Funding
- NCRR NIH HHS [P41 RR008119, RR-08119] Funding Source: Medline
Ask authors/readers for more resources
We describe a new approach to making luminophores that display long emission wavelengths, long decay times, and high quantum yields. These luminophores are covalently linked pairs with a long-lifetime resonance-energy-transfer (RET) donor and a long-wavelength acceptor. The donor was a ruthenium (Ru) metal-ligand complex. The acceptor was the Texas Red. The donor and acceptor were covalently linked by polyproline spacers. The long-lifetime donor results in a long-lived component in the acceptor decay, which is due to RET. Importantly, the quantum yield of the luminophores approaches that of the higher quantum yield acceptor, rather than the lower quantum yield typical of metal-ligand complexes. The emission maxima and decay time of such tandem luminophores can be readily adjusted by selection of the donor, acceptor, and distance between them. Luminophores, with these useful spectral properties can also be donor-acceptor pairs brought into close proximity by some biochemical association reaction. Luminophores with long-wavelength emission and long lifetimes can have numerous applications in biophysics, clinical diagnostics, DNA analysis, and drug discovery.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available