4.7 Article

Cloning of parsley flavone synthase I

Journal

PHYTOCHEMISTRY
Volume 58, Issue 1, Pages 43-46

Publisher

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0031-9422(01)00191-1

Keywords

Petroselinum crispum; Apiaceae; flavonoid biosynthesis; flavone synthase I cloning; 2-oxoglutarate-dependent dioxygenase; heterologous expression

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A cDNA encoding flavone synthase I was amplified by RT-PCR from leaflets of Petroselinum crispum cv. Italian Giant seedlings and functionally expressed in yeast cells. The identity of the recombinant, 2-oxoglutarate-dependent enzyme was verified in assays converting (2S)-naringenin to apigenin. (C) 2001 Elsevier Science Ltd. All rights reserved.

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