Journal
JOURNAL OF VIROLOGY
Volume 75, Issue 17, Pages 8016-8020Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.75.17.8016-8020.2001
Keywords
-
Categories
Funding
- NIAID NIH HHS [AI36555, AI399975-01] Funding Source: Medline
Ask authors/readers for more resources
Targeted stable transduction of specific cells is a highly desirable goal for gene therapy applications. We report an efficient and broadly applicable approach for targeting retroviral vectors to specific cells. We find that the envelope of the alphavirus Sindbis virus can pseudotype human immunodeficiency virus type 1- and murine leukemia virus-based retroviral vectors. When modified to contain the Fc-binding domain of protein A, this envelope gives a significant enhancement in specificity in combination with antibodies specific for HLA and CD4 relative to that without antibody. Unlike previous targeting strategies for retroviral transduction, the virus titers are relatively high and stable and can be further increased by ultracentrifugation. This study provides proof of principle for a targeting strategy that would be generally useful for many gene therapy applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available