Journal
JOURNAL OF GENERAL VIROLOGY
Volume 82, Issue -, Pages 2169-2172Publisher
SOC GENERAL MICROBIOLOGY
DOI: 10.1099/0022-1317-82-9-2169
Keywords
-
Categories
Ask authors/readers for more resources
To study the neutralizing epitopes of influenza B virus Victoria group strains, two monoclonal antibodies (MAbs) were used to select antigenic variants of the virus. MAbs 10B8 and 8E6 were found to react with B/Victoria group strains in three tests, peroxidase-antiperoxidase staining, haemagglutination inhibition and neutralization tests; no reactivity with B/Yamagata group strains was observed. Analysis of the deduced amino acid sequences of 10B8-induced variants identified a single amino acid deletion at residue 165 or 170, as well as a single amino acid substitution at residues 164(Asp --> Tyr), 165 (Asn --> Ser or Thr) or 203 (Lys --> Thr or Asn). A single amino acid substitution at residue 241 (Pro --> Ser) was observed in 8E6-induced variants. Three-dimensional analysis showed that the epitopes for both MAbs were situated in close proximity to each other. Since B/Yamagata group strains are characterized by amino acid deletions at residues 164-166, the epitope for MAb 10B8 is strictly specific for B/ Victoria group strains.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available