Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 97, Issue 1-2, Pages 113-123Publisher
ELSEVIER
DOI: 10.1016/S0166-0934(01)00339-1
Keywords
bovine viral diarrhea virus; hepatitis C virus; viral genome
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A chimeric cDNA genome was constructed in which the core, E1 and E2 genes of hepatitis C virus (HCV) replaced the core, E-rns, E1 and E2 genes of bovine viral diarrhea virus (BVDV). High levels of HCV structural proteins were expressed in a small number of human or bovine cells following transfection with chimeric RNA. However, in one cell line, bovine embryonic trachea cells [EBTr(A)], the number of cells expressing HCV proteins increased to greater than 70% following serial passage of culture medium. These cells were persistently infected with a non-cyto pathogenic BVDV helper virus. In these cells, the chimeric genome was packaged into infectious particles that accumulated in the culture medium at a titer as high as 10(7)-10(9) genome equivalents per mi. The virus particles were pseudotypes, because they were neutralized by anti-BVDV but not by anti-HCV. (C) 2001 Elsevier Science B.V. All rights reserved.
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