Journal
HYPERTENSION
Volume 38, Issue 3, Pages 650-654Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/hy0901s1.095011
Keywords
angiotensin-converting enzyme; angiotensin; polymorphism; neutral endopeptidase
Categories
Ask authors/readers for more resources
Angiotensin I is a substrate for both ACE and for neutral endopeptidase 24.11 (NEP). We hypothesized that high ACE expression is related to low NEP activity. Accordingly, circulating and tissue NEP and ACE activities were measured by fluorometry in homozygous rats (FO and 172) for the Lewis microsatellite allele (LL, low ACE) and for the Brown Norway microsatellite allele (BB, high ACE). Plasma, lung, and aortic ACE activities in F-0 and F-2 were higher in BB rats than in LL rats (P <0.01), whereas left ventricular ACE activity was similar in both genotypes. In contrast, NEP activity in the LL group was higher in the serum, aorta, and lungs in F-0 and F-2 homozygous (P <0.05). Plasma ACE activity was inversely correlated with serum (r=-0.6 and -0.598 in F-0 and F-2, respectively; P <0.03) and lung NEP activities (r=-0.77 in F-0 and r=-0.59 in F-2, P <0.01). Aortic ACE and NEP activities were also correlated (r=-0.696 and -0.584 in F-0 and F-2, respectively; P <0.03). In conclusion, genetically determined high ACE expression in rats is inversely related to tissue NEP activity, which could determine lower angiotensin-(1-7) tissue levels.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available