Journal
INFECTION AND IMMUNITY
Volume 69, Issue 9, Pages 5671-5678Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.69.9.5671-5678.2001
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Funding
- NCI NIH HHS [P30 CA013330, CA13330] Funding Source: Medline
- NHLBI NIH HHS [R01 HL059842, HL59842] Funding Source: Medline
- NIAID NIH HHS [AI33142, R01 AI033142, AI333774, R37 AI033142, R01 AI033774, N01-AI-75320] Funding Source: Medline
- PHS HHS [1K08A101691, T32A107501] Funding Source: Medline
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The outermost layer of Mycobacterium tuberculosis contains two major polysaccharides, arabinomannan (AM) and glucan (GC). We studied the in vitro and in vivo expression of an M. tuberculosis AM antigen using monoclonal antibody (MAb) 9d8 (2a), an isotype-switched variant of the immunoglobulin G3 (IgG3) MAb 9d8. MAb 9d8 had been previously shown to bind M. tuberculosis AM and the M. tuberculosis surface. Our in vitro experiments showed that MAb 9d8(2a) hound strongly to whole-cell M. tuberculosis Erdman but not to the CDC 1551 strain grown in medium for an extended period. However, AM antigen was detected in the culture supernatant of both strains, and its concentration increased in a time-dependent manner. The detection of AM antigen from both strains was decreased in the presence of Tween 80. In mice infected with M. tuberculosis Erdman, AM antigen accumulated in organ homogenates concomitant to an increase in bacterial organ burden and an increase in IgG and IgM titer to AM. These results (i) indicate that the surface expression of AM during in vitro growth changes with culture age, is strain dependent, and is affected by the presence of Tween 80 in the culture media; (ii) show that AM is produced by bacteria growth in vivo; and (iii) demonstrate that the amount of in vivo-detected AM can be dependent on the number of bacteria in the infected organ.
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