Journal
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
Volume 78, Issue 9, Pages 949-951Publisher
SPRINGER
DOI: 10.1007/s11746-001-0369-x
Keywords
amylase; cellulase; enzyme-assisted aqueous extraction; protease; rice bran oil
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In the present study, rice bran oil was extracted by enzyme-assisted aqueous extraction under optimized aqueous extraction conditions using mixtures of Protizyme((TM)) (protease; Jaysons Agritech Pvt. Ltd., Mysore, India), Palkodex((TM)) (alpha -amylase; Maps India Ltd., Ahmedabad, India), and cellulase (crude cellulase; Central Drug House, Delhi, India). The optimal conditions used were: mixtures of amylase (80 U), protease (368 U), and cellulase (380 U), with 10 g of rice bran in 40 mL distilled water, pH 7.0, temperature 65 degreesC, extraction time 18 h with constant shaking at 80 rpm. Centrifugation of the mixture at 10,000 x g for 20 min yielded a 77% recovery of the oil.
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