Journal
ANALYST
Volume 140, Issue 10, Pages 3565-3572Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4an01768d
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Funding
- NSW Cancer Council
- NHMRC [APP1045841]
- NHMRC CDA
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Efficient isolation strategies not based on epithelial biomarker expression are required to enable nonbiased enrichment of circulating tumor cells (CTCs). CTCs undergoing epithelial-mesenchymal transition (EMT) may be prognostically relevant, and importantly are not detected with conventional epithelial based approaches such as CellSearch (R). A method for the non-biased isolation of cancer cells within a peripheral blood sample utilizing microfluidic mixing PDMS devices functionalized with anti-CD45 is reported. The introduction of micro and nanoscale roughness using a single step treatment with sulfuric acid significantly increases the binding yield of white blood cells (WBCs) to the anti-CD45 conjugated surfaces. Up to 99.99% WBC depletion is achieved with a tumor cell recovery yield of 50%. This high level of CTC enrichment is expected to facilitate the detailed characterization of CTCs using for instance, imaging flow cytometry as demonstrated here.
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