Transient Inactivation of the Ventral Tegmental Area Selectively Disrupts the Expression of Conditioned Place Preference for Pup- but Not Cocaine-Paired Contexts

The Ventral tegmental area (VTA) plays a critical role in motivated behavior However. it remains unclear whether Intact VTA function Is necessary for motivated behavior to seek contexts repeatedly paired with natural stimuli and/or pharmacological Stimuli In the present Study. conditioned place preference (CPP) was induced with highly salient natural or drug stimuli attributed with strong incentive-motivational Value in each of 2 female models Postpartum females were conditioned to associate one unique context in the CPP apparatus with young offspring (pups) and a second context with I neutral Stimulus. and virgin females were conditioned to associate unique contexts with cocaine (5 mg/kg ip) and saline injections Immediately before CPP testing each female received a microinfusion of bupivacaine bilaterally into the VTA to transiently inactivate the region, subjects were also tested after saline microinfusion into the VTA Postpartum females' preference for the pup-paired context was abolished by VTA Inactivation bill was restored to high control levels after saline microinfusion In separate tests. VTA Inactivation also reduced motivated PLIP licking and pup retrieval in postpartum females, suggesting that intact VTA function is required for the expression of both pup CPP and motivated pup-directed behaviors Cocaine CPP remained Unaffected by VTA Inactivation. Locomotion was not affected by VTA microinfusions but was severely impaired by bupivacaine microinfusions into the substantia nigra. We concluded that the VTA is differentially Involved in the expression of conditioned preference for contexts paired with pups. a salient natural stimulus, and contexts paired with cocaine