Journal
MAGNETIC RESONANCE IN MEDICINE
Volume 46, Issue 3, Pages 424-429Publisher
JOHN WILEY & SONS INC
DOI: 10.1002/mrm.1209
Keywords
magnetic resonance imaging; retinal projection; manganese; neuroanatomic tract tracing
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This study describes the neuroaxonal tracing of the visual pathway in the living rat using high-resolution T-1-weighted 3D gradient-echo MRI (195 x 195 x 125 mum(3)) at 8, 24, 48, and 72 h after intraocular Mn2+ injection (0.1 mul of 1 M aqueous MnCl2). Best results were obtained at 24 h postinjection, revealing a continuous pattern of anterograde labeling from the retina, optic nerve, and chiasm to the contralateral optic tract, the dorsal and ventral lateral geniculate nucleus, the superior colliculus and its brachium, the olivary pretectal nucleus, the nucleus of the optic tract, and the suprachiasmatic nucleus. These results underline the feasibility of repeated MRI tract tracing in living animals after a single injection of Mn2+. The approach is expected to advance studies of neuroaxonal function in behaving animals with special emphasis on applications in developmental neurobiology. (C) 2001 Wiley-Liss, Inc.
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