Journal
BIOTECHNOLOGY LETTERS
Volume 23, Issue 18, Pages 1455-1462Publisher
SPRINGER
DOI: 10.1023/A:1011623509335
Keywords
biofuel; cellulase; cellulose; endoglucanase; ethanol
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A derivative of Klebsiella oxytoca M5A1 containing chromosomally integrated genes for ethanol production from Zymomonas mobilis (pdc, adhB) and endoglucanase genes from Erwinia chrysanthemi (celY, celZ) produced over 20 000 U endoglucanase l(-1) activity during fermentation. In combination with the native ability to metabolize cellobiose and cellotriose, this strain was able to ferment amorphous cellulose to ethanol (58-76% of theoretical yield) without the addition of cellulase enzymes from other organisms.
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