4.6 Article

Function of YidC for the insertion of M13 Procoat protein in Escherichia coli -: Translocation of mutants that show differences in their membrane potential dependence and Sec requirement

Journal

JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 276, Issue 37, Pages 34847-34852

Publisher

AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M105793200

Keywords

-

Funding

  1. NIGMS NIH HHS [GM63862] Funding Source: Medline

Ask authors/readers for more resources

The membrane insertion of the Sec-independent M13 Procoat protein in bacteria requires the membrane electrochemical potential and the integral membrane protein YidC. We show here that YidC is involved in the translocation but not in the targeting of the Procoat protein, because we found the protein was partitioned into the membrane in the absence of YidC. YidC can function also to promote membrane insertion of Procoat mutants that insert independently of the membrane potential, proving that the effect of YidC depletion is not due to a dissipation of the membrane potential. We also found that YidC is absolutely required for Sec-dependent translocation of a long periplasmic loop of a mutant Procoat in which the periplasmic region has been extended from 20 to 194 residues. Furthermore, when Sec-dependent membrane proteins with large periplasmic domains were overproduced under YidC-limited conditions, we found that the exported proteins pro-OmpA and pre-peptidoglycan-associated lipoprotein accumulated in the cytoplasm. This suggests for Sec-dependent proteins that YidC functions at a late stage in membrane insertion, after the See translocase interacts with the translocating membrane protein. These studies are consistent with the understanding that YidC cooperates with the See translocase for membrane translocation and that YidC is required for clearing the protein-conducting channel.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available