Journal
FEBS LETTERS
Volume 505, Issue 2, Pages 264-268Publisher
WILEY
DOI: 10.1016/S0014-5793(01)02836-8
Keywords
ceramide; Bax; Bcl-xL; apoptosis; caspase
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Ceramide is an important lipid messenger involved in mediating a variety of cell functions including apoptosis. In this study, we show that antisense bax inhibits cytochrome c release, poly(ADP-ribose)polymerase cleavage and cell death induced by ceramide in HL-60 cells. In addition, ceramide induces translocation of Bax to mitochondria. The addition of the broad spectrum caspase inhibitor zVAD-fmk prevented ceramide-induced apoptotic cell death but did not inhibit translocation of Bax and mitochondrial cytochrome c release. Furthermore, ceramide inhibits the expression of the antiapoptotic protein Bcl-xL with an increase in the ratio of Bax to Bcl-xL. These data provide direct evidence that Bax plays an important role in regulating ceramide-induced apoptosis. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
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