Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 57, Issue 11, Pages 2197-2211Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S1386-1425(01)00493-0
Keywords
rare earth cryptates; time resolved fluorescence; fluorescence resonance energy transfer; molecular interactions
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A homogeneous assay technology using time resolved fluorescence and fluorescence resonance energy transfer is described. A new class of fluorescent complexes, the cryptates, have been used as fluorescent donor with cross-linked allophycocyanin as acceptor. This new donor/acceptor shows an exceptionally high Forster distance R-0 of 9 nm. This allows to build up a set of strategies to probe the interactions of biomolecules in biology, particularly for high throughput screening applications. In this article, we describe the basics of the technology and review applications developed for studying different key molecular interactions involved in cellular processes. (C) 2001 Elsevier Science B.V. All rights reserved.
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