Journal
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Volume 393, Issue 2, Pages 308-315Publisher
ELSEVIER SCIENCE INC
DOI: 10.1006/abbi.2001.2499
Keywords
CYP mRNA; quantitative RT-PCR; CYP activities; specific substrates; human liver; CYP regulation
Categories
Ask authors/readers for more resources
CYP activity and protein contents have been measured in human liver using different techniques. In contrast, CYP m.RNA data are scarce and the relationships between CYP mRNA contents and activities have not been established. These studies deserve further attention because mRNA determinations by RT-PCR require a very small amount of material (e.g., liver needle biopsy) and could provide important data regarding CYP expression regulation. In this study we measured in 12 human liver samples the mRNA contents of 10 CYPs by quantitative RT-PCR and the metabolic activities using specific substrates. m-RNA contents and activities showed high correlation coefficients for CYP1A1, CYP1A2, CYP3A4, CYP2B6, and CYP2B6 (0.96, 0.94, 0.69, 0.61, and 0.52, respectively), but no significant correlations were found for CYP2C9, CYP2A6, and CYP2E1. The results suggest that the regulation of CYP1A1, CYP1A2, CYP3A4, CYP2136, and CYP2B6 expression is essentially pretranslational and that their mRNA levels could allow a good estimate of their activity. (C) 2001 Academie Press.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available