Journal
JOURNAL OF EXPERIMENTAL MEDICINE
Volume 194, Issue 6, Pages 707-717Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.194.6.707
Keywords
cytotoxic T lymphocytes; peripheral tolerance; T cell activation; B7; IFN-gamma
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Funding
- NCI NIH HHS [CA57855] Funding Source: Medline
- NIDDK NIH HHS [DK50824] Funding Source: Medline
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Not all T cells specific for autoantigens are eliminated in the thymus, and therefore alternate mechanisms are required to prevent potentially autoreactive T cells from developing into effectors. Adoptive transfer of CD8(+) T cells from influenza hemagglutinin-specific Clone 4 TCR, transgenic mice into mice that express hemaglutinin in the pancreatic islets results in tolerance. This is preceded by activation of Clone 4 T cells that encounter antigen cross-presented in the draining lymph nodes of the pancreas. In this report we compare the phenotype, function, and costimulatory requirements of Clone 4 T cells activated by endogenous self-antigen, with Clone 4 T cells stimulated by influenza virus. The cells undergoing tolerance upregulate both CD69 and CD44, yet only partially downregulate CD62L, and do not express CD49d or CD25. Most importantly, they lack the ability to produce inteiferon-gamma in response to antigen and show no cytolytic activity. Clone 4 T cells disappear after several cycles of division, apparently without leaving the site of initial activation. Surprisingly, despite the fact that such stimulation occurs through recognition of antigen that is cross-presented by a professional antigen-presenting cell, we find this activation is not dependent on costimulation through CD28. These data demonstrate that the recognition by naive CD8(+) T cells of cross-presented self-antigen results in localized proliferation and deletion, without the production of effector cells.
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