Journal
FEBS LETTERS
Volume 505, Issue 3, Pages 383-388Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(01)02853-8
Keywords
host cell receptor for Trypanosoma cruzi; gp83 trans-sialidase ligand; host cell receptor for gp83 trans-sialidase; Trypanosoma cruzi ligand; Trypanosoma cruzi attachment and entry
Funding
- NCRR NIH HHS [RR03031] Funding Source: Medline
- NHLBI NIH HHS [HL-03156, HL-03149] Funding Source: Medline
- NIAID NIH HHS [AI-25637, AI-07281] Funding Source: Medline
- NIGMS NIH HHS [GM08037] Funding Source: Medline
Ask authors/readers for more resources
We purified a soluble gp83 trans-sialidase (gp83-TSA), from phospholipase C-treated Trypanosoma cruzi trypomastigote membranes, which binds to myoblasts, fibroblasts and macrophages to mediate trypanosome entry. Myoblasts display a single class of receptors for the gp83-TSA present at 4 X 10(4) per myoblast with a K-d of 8 nM. Monovalent Fab fragments of the monoclonal antibody 4A4 specific for gp83-TSA inhibit gp83-TSA binding to myoblasts, fibroblasts and macrophages, block the trypanosomes from attaching to and entering these cells and neutralize T. cruzi infection in BALB/c mice. This is the first demonstration that gp83-TSA is a ligand that T. cruzi uses to attach to cells. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
