Journal
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
Volume 1520, Issue 3, Pages 187-194Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0167-4781(01)00268-8
Keywords
apoptosis; Boo/Diva; cervical cancer
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Funding
- NCI NIH HHS [R01 CA20068, 5 K08 CA79509-03, P01 CA42792] Funding Source: Medline
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Mouse Boo/Diva is an ovary-specific member of the Bcl-2 family identified through homology with the avian cell death antagonist NR13, We identified a human orthologue of Boo/Diva, which is highly conserved between mouse and human and related to avian NR13. Human Boo/Diva is also expressed in human liver and kidney in addition to the ovary. We found that green fluorescence protein (EGFP)-tagged Boo/Diva was not exclusively localized to mitochondria before the induction of apoptosis. However, EGFP-Boo/Diva translocated to mitochondria in the process of apoptosis induced by vincristine. a microtubule-interfering agent. Overexpression of human Boo/Diva promoted cell death in HeLa and 293 cells. The cell death antagonist Bcl-XL interacts with Boo. but is unable to protect 293 cells from Boo/ Diva-induced cell death. Finally, we mapped human Boo/Diva to chromosome 15q21, a locus known to be related to human cervical cancer. Moreover. we found that genomic DNAs of three of 24 human cervical cancer samples display deletions Nk within their Boo/Diva genes. This result suggests a role for human Boo/Diva in the pathogenesis of cervical cancer. (C) 2001 Elsevier Science B.V. All rights reserved.
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