Journal
JOURNAL OF APPLIED POLYMER SCIENCE
Volume 81, Issue 13, Pages 3327-3337Publisher
JOHN WILEY & SONS INC
DOI: 10.1002/app.1789
Keywords
bovine serum albumin; poly(N-vinyl pyrrolidone-alt-maleic anhydride); kinetics by H-1 NMR; charge transfer complex radical polymerization; light scattering
Categories
Ask authors/readers for more resources
Alternate N-vinyl pyrrolidone/maleic anhydride (NVPMA) copolymers were obtained by radical solution polymerization in dioxane with various MA contents in the monomer feed. The conversion of each monomer was monitored by proton nuclear magnetic resonance spectroscopy (H-1 NMR), and the kinetics investigation showed that both monomers had identical polymerization rates if both monomers were present in the reaction mixture. The presence of excess NVP in the polymerization medium increased the kinetics of the polymerization and the molar masses of the resulting polymers. This increase was attributed to a cosolvent effect due to NVP, which is a better solvent for the polymer than dioxane. The hydrolysis rate constant of the polymers increased with pH, and NVPMA copolymers were more prone to hydrolysis (by a factor 10) than the methyl vinyl ether ones. Finally, the immobilization of bovin serum albumin (BSA) was investigated. A 25 mM phosphate buffer (pH 5.5) was the best medium to covalently bind 5 BSA molecules onto a 29 kDa NVPMA copolymer and 13 BSA molecules onto a 58 kDa sample, with grafting efficiencies > 90%. Noncovalent interactions with the hydrolyzed form of the polymer and BSA occured at pHs lower than the isoelectric point of BSA, and the resulting complexes were insoluble in water. (C) 2001 John Wiley & Sons, Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available