Journal
JOURNAL OF CHROMATOGRAPHY B
Volume 761, Issue 2, Pages 167-175Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4347(01)00326-7
Keywords
derivatization; LC; colistin; 9-fluorenylmethyl chloroformate
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A simple, selective and sensitive high-performance liquid chromatographic (HPLC) method is described for the determination of colistin in human plasma. Derivatization with 9-fluorenylmethyl chloroformate was performed in the same solid-phase extraction C-18 cartridge used for sample pre-treatment, followed by reversed-phase HPLC with fluorimetric detection. Quantification was achieved using the ratio of the summed peak areas of colistin A and B derivatives to that of the derivative of netilmicin (internal standard), Linear calibration curves were obtained within the concentrations of colistin sulfate from 0.10 to 4.0 mg/l in plasma. Accuracy was within 10% and reproducibility (RSD) was less than 10%. Crown copyright. (C) 2001 Published by Elsevier Science B.V. All rights reserved.
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