Alterations of HLA class I and II antigen expression in preinvasive, invasive and metastatic cervical cancers

HLA expression is altered in a large variety of human cancers. We performed immunohistochemical staining on tissues,from. normal, preinvasive, invasive and metastatic cervical cancer tissues using anti-HLA class 1. or class If antibody. In tissues from normal squamous epithelium, carcinoma in situ (CIS) and microinvasive carcinoma (MIC), the expressions of HLA-B, C heavy chains and class II heavy chain were significantly decreased as disease progressed. When the expression patterns were compared between primary and metastatic squamous cell carcinoma (SCC) lesions, statistically significant down-regulation of HLA class I and class II antigen In metastatic lesions was observed. The rates of HLA-B, C heavy chains and class If heavy chain expressions were all significantly down-regulated compared to the down-regulation rate of class I beta2-microglobulin (beta 2m) in invasive squamous lesions, and the expressions of class II heavy chain in metastatic lesions was,decreased further than that in primary lesions. Unlike SCC, the degree of HLA class I and class II loss was not. evident as disease progressed in early stage of adenocarcinoma. In invasive adenocarcinoma lesions, only the expression of HLA-B, C heavy chains was decreased and no differences were seen in HLA-B, C heavy chain expression patterns between primary and met;. astatic lesions. These results, suggest that alterations of HLA class. I and II expressions seem. to occur at a particular step in cervical cancer, development and depend on, tissue types., when the tumor becomes invasive and starts to, metastasize.