Journal
MOLECULAR PHYSICS
Volume 99, Issue 19, Pages 1689-1699Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/00268970110063917
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In this study, the aromatic-thiol pi hydrogen bonding and phenylalanine-cysteine side chain interactions are characterized through both molecular orbital calculations on a C6H6-HSCH3 model complex and database analyses of 609 X-ray protein structures. The aromatic-thiol pi hydrogen bonding interaction can achieve a stabilization energy of 2.60 kcal mol(-1), and is stronger than the already documented aromatic-hydroxyl and aromatic-amino hydrogen bonds. However, the occurrence of the aromatic-thiol hydrogen bond is rather rare in proteins. This is because most of the thiol groups participate in the formation of either disulphide bonds or stronger S-H . . .O (or N) 'normal' hydrogen bonds in a protein environment. Interactions between the side chains of phenylalanine and cysteine residues are characterized as the phenyl( Phe)-(HSCH2-)(Cys) interaction. The bonding energy for such interactions is approximately 3.71 kcal mol(-1) and is achieved in a geometric arrangement with an optimal phenyl(Phe)-(HS-)(Cys) pi -type hydrogen bonding interaction. The interaction is very sensitive to the orientation of the two lone electron pairs on the sulphur atom relative to the p electron cloud of the phenyl ring. Accordingly, the interaction configurations that can accomplish a significant bonding energy exist only within a narrow configurational space. The database analysis of 609 experimental X-ray protein structures demonstrates that only 268 of the 1620 cysteine residues involve such phenylalanine-cysteine side chain interactions. Most of these interactions occur in the form of pi (aromatic)-lone pair(sulphur) attractions, and correspond to a bonding energy less than 1.5 kcal mol(-1). A few were identified as the aromatic-thiol hydrogen bond with a bonding energy of 2.0-3.6 kcal mol(-1).
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