Journal
JOURNAL OF IMMUNOLOGY
Volume 167, Issue 7, Pages 3953-3961Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.167.7.3953
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Funding
- NIAID NIH HHS [AI-20943] Funding Source: Medline
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Time-lapsed video microscopy and confocal imaging were used to study the migration of wild-type (WT) and mitogen-activated protein kinase-activated protein kinase 2 (MK2)(-/-) mouse neutrophils in Zigmond chambers containing fMLP gradients. Confocal images of polarized WT neutrophils showed an intracellular gradient of phospho-MK2 from the anterior to the posterior region of the neutrophils. Compared with WT neutrophils, MK2(-/-) neutrophils showed a partial loss of directionality but higher migration speed. Immunoblotting experiments showed a lower protein level of p38 mitogen-activated protein kinase and a loss of fMLP-induced extracellular signal-related kinase phosphorylation in MK2(-/-) neutrophils. These results suggest that MK2 plays an important role in the regulation of neutrophil migration and may also affect other signaling molecules.
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