Journal
MOLECULAR BIOTECHNOLOGY
Volume 19, Issue 2, Pages 201-203Publisher
HUMANA PRESS INC
DOI: 10.1385/MB:19:2:201
Keywords
RNA isolation; CTAB; fruit tissue; bilberry; Vaccinium myrtillus
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A simple and efficient method is described for isolating high quality RNA from bilberry fruit. The procedure is based on the use of hexadecyltrimethyl ammonium bromide (CTAB), polyvinylpyrrolidone (PVP), and beta -mercaptoethanol. in an extraction buffer in order to eliminate the polysaccharides and prevent the oxidation of phenolic compounds. This method is a modification of the one described for pine trees, and yields high-quality RNA suitable for cDNA based methodologies. This method is applicable for a variety of plant tissues.
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