Journal
CURRENT OPINION IN BIOTECHNOLOGY
Volume 12, Issue 5, Pages 473-480Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S0958-1669(00)00248-2
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Site-specific recombinases have become powerful tools for the targeted integration of transgenes into defined chromosomal loci. They have been successfully used both to achieve predictable gene expression in cell culture and for the systematic creation of transgenic animals. A recent improvement of this method, the recombinase-mediated cassette exchange procedure (RMCE), permits expression in the absence of any co-expressed selection marker gene.
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