Journal
MOLECULAR THERAPY
Volume 4, Issue 4, Pages 372-374Publisher
CELL PRESS
DOI: 10.1006/mthe.2001.0462
Keywords
AAV5 purification; sialic acid; mucin; affinity column
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Funding
- NIDDK NIH HHS [P30 DK47757] Funding Source: Medline
- Telethon [371/B] Funding Source: Medline
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Here we describe a single-step affinity column for purification of vectors based on adeno-associated virus type 5 (AAV5). A sialic-acid-rich protein called mucin was covalently attached to Sepharose and was found to bind AAV5 vectors. Elution with high salt efficiently recovered highly active vectors of greater purity than what is achieved with CsCl2 sedimentation.
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