Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 67, Issue 10, Pages 4896-4900Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.67.10.4896-4900.2001
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We propose a new method for the fast separation and detection of (beta -glucosidases in environmental samples. With this approach, beta -glucosidases extracted from bacteria are evidenced by substrate-incorporated capillary electrophoresis (CE zymography) and their kinetic parameters can be determined by repeated injections using different substrate concentrations. Preliminary results obtained with natural bacterial communities from the coastal North Sea suggest that the diversity of beta -glucosidases in the marine environment might be much higher than previously observed.
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