Journal
MOLECULAR AND CELLULAR BIOLOGY
Volume 21, Issue 19, Pages 6668-6680Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.21.19.6668-6680.2001
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Funding
- NCI NIH HHS [T32 CA09385, T32 CA009385] Funding Source: Medline
- NIGMS NIH HHS [R01 GM052461, R56 GM052461, GM52461] Funding Source: Medline
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We have performed a systematic structure-function analysis of Saccharomyces cerevisiae TAF25, an evolutionarily conserved, single-copy essential gene which encodes the 206-amino-acid TAF25p protein. TAF25p is an integral subunit of both the 15-subunit general transcription factor TFIID and the multisubunit, chromatin-acetylating transcriptional coactivator SAGA. We used hydroxylamine mutagenesis, targeted deletion, alanine-scanning mutagenesis, high-copy suppression methods, and two-hybrid screening to dissect TAF25. Temperature-sensitive mutant strains generated were used for coimmunoprecipitation and transcription analyses to define the in vivo functions of TAF25p. The results of these analyses show that TAF25p is comprised of multiple mutable elements which contribute importantly to RNA polymerase II-mediated mRNA gene transcription.
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