Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 67, Issue 10, Pages 4926-4929Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.67.10.4926-4929.2001
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The genetic diversity of Borrelia burgdorferi sensu lato was assessed in individual adult Ixodes ricinus ticks from Europe by direct PCR amplification of spirochetal DNA followed by genospecies-specific hybridization. Analysis of mixed infections in the ticks showed that B. garinii and B. valaisiana segregate from B. afzelii. This and previous findings suggest that host complement interacts with spirochetes in the tick, thereby playing an important role in the ecology of Lyme borreliosis.
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