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Vectors to express foreign genes and techniques to monitor gene expression in Pseudomonads

Journal

CURRENT OPINION IN BIOTECHNOLOGY
Volume 12, Issue 5, Pages 439-445

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/S0958-1669(00)00242-1

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Improved tools for Pseudomonas research include small, broad-host-range vectors that allow regulated expression from the lac operon and T7 promoters whose biology is well understood and adaptable to many bacteria. To facilitate studies on gene regulation, tracking and monitoring of bacteria in diverse environments, and the construction of biosensors, various reporter genes with versatile assay formats have been developed that can be delivered on plasmid, transposon and integration-proficient vectors.

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