Journal
EXPERIMENTAL DERMATOLOGY
Volume 10, Issue 5, Pages 329-336Publisher
WILEY
DOI: 10.1034/j.1600-0625.2001.100505.x
Keywords
telomerase; telomere; T lymphocyte subset; CLA(+) cell
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We have recently demonstrated that telomerase activity is increased and telomere length shortened in lymphocytes from peripheral blood of patients with cutaneous T-cell lymphoma. In order to determine which cell type has increased telomerase activity and shortened telomere length, CD4(+), CD8(+), CLA(+) CD3(+) and CLA(-) CD3(+) T cells were isolated from peripheral blood of 25 patients, including 15 patients with mycosis fungoides and 10 patients with parapsoriasis. Eleven healthy individuals were used as controls; CD19(+) B cells were separated from each individual as an internal control. The results showed that the increased telomerase activity was significantly predominating in the CD4(+) T-cell subset. Significantly shortened telomere length was found in CD4(+) and CD8(+) T-cell subsets from the patients compared with the same cell subsets obtained from healthy individuals. However, no difference was observed between the subsets; CD19(+) B cells collected from patients and healthy control individuals had similar telomerase activity and telomere length which was significantly different from the values found in T cells. The telomere length was significantly shorter in CLA(+) CD3(+) subset than in CLA(-) CD3(+) subset. Interestingly, increased telomerase activity and shortened telomere length was also detected in CD4(+) T cells from patients with parapsoriasis indicating that alteration of telomerase activity and telomere length in CD4(+) T cells is an early event in the pathogenesis of cutaneous T-cell lymphoma. Thus, the results indicate that a significant high level of telomerase activity and shortened telomere length frequently occur in T cells of patients with CTCL and may reflect tumorigenesis.
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