Journal
JOURNAL OF NEUROSCIENCE
Volume 21, Issue 20, Pages 8034-8042Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.21-20-08034.2001
Keywords
adaptor protein; synaptic vesicle; AP-3; endosome; brain; neuronal isoforms
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Funding
- NIDA NIH HHS [DA10154] Funding Source: Medline
- NINDS NIH HHS [NS09878] Funding Source: Medline
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Heterotetrameric adaptor complexes vesiculate donor membranes. One of the adaptor protein complexes, AP-3, is present in two forms; one form is expressed in all tissues of the body, whereas the other is restricted to brain. Mice lacking both the ubiquitous and neuronal forms of AP-3 exhibit neurological disorders that are not observed in mice that are mutant only in the ubiquitous form. To begin to understand the role of neuronal AP-3 in neurological disease, we investigated its function in in vitro assays as well as its localization in neural tissue. In the presence of GTP gammaS both ubiquitous and neuronal forms of AP-3 can bind to purified synaptic vesicles. However, only the neuronal form of AP-3 can produce synaptic vesicles from endosomes in vitro. We also identified that the expression of neuronal AP-3 is limited to varicosities of neuronal-like processes and is expressed in most axons of the brain. Although the AP-2/clathrin pathway is the major route of vesicle production and the relatively minor neuronal AP-3 pathway is not necessary for viability, the absence of the latter could lead to the neurological abnormalities seen in mice lacking the expression of AP-3 in brain. In this study we have identified the first brain-specific function for a neuronal adaptor complex.
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